In this
experiment, two hypotheses were proposed for the process of photosynthesis in
spinach leaf chloroplasts under the effect of herbicide (a drug that affects
electron transport). In order to test the hypothesis, 1 experiment cuvette and
4 controls – each containing different ratios of chloroplast solution, Na3PO4 (sodium
phosphate buffer), herbicide, DCPIP (and reduced DCPIP), and water -- were tested. By examining the
recorded absorbance data (Table 1), Hypothesis 1 was supported: herbicide
stopped photosynthesis before Pq, having no effect on the color of DCIP and
thus remaining blue (oxidized form). The absorbance values show that the significance was null: there were no significant
changes/decreases in absorbance throughout the 9 rounds, rather, the values showed relativity -- fluctuating only about a range of 0.001 – 0.2
(not significant). Since the absorbance values did not significantly decrease
(meaning, a change in color of DCPIP from blue to clear), it can be
hypothesized that herbicide stopped photosynthesis (specifically, affected the
electron transport chain) before Pq, thus, a plug, preventing DCPIP from
obtaining electrons and turning clear. The support of Hypothesis 1 allows for the
refutation of Hypothesis 2 – herbicide stopping photosynthesis after Pq and
thus resulting in DCPIP changing color from blue to clear. Based on objective
absorbance data staying relatively constant throughout the test rounds,
Hypothesis 1 was supported and Hypothesis 2 was refuted in this experiment.
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I have identified 4 nominalizations.
My character(s) include my hypothesis being supported/refuted through experimental data.
The purpose of this paragraph is to support my hypothesis of the effect of herbicide on the process of photosynthesis.
Topic Position Stress Position (indicated by /)
1. The absorbance values indicate there was no significance in the results / since there were no significance decreases in absorbance.
2. The values were relative to one another; / the values in between rounds fluctuated only about a range of 0.001 - 0.2.
3. Pq plugged the electron transport chain / by preventing DCIPIP from obtaining electrons.
4. The support of Hypothesis 1 inherently refutes Hypothesis 2 - / meaning that herbicide stopped photosynthesis after Pq which resulted in DCIPIP changing color from blue to clear since it was able to steal electrons.
New paragraph: In this experiment, the effect of herbicide was tested on spinach leaf chloroplasts by measuring absorbance values during the process of photosynthesis. Two hypotheses were proposed: herbicide will affect the electron transport chain either before or after Pq. In order to test the hypothesis, 1 experiment cuvette and 4 controls – each containing different ratios of chloroplast solution, Na3PO4 (sodium phosphate buffer), herbicide, DCPIP (and reduced DCPIP), and water -- were tested. By examining the recorded absorbance data (Table 1), Hypothesis 1 was supported: herbicide stopped photosynthesis before Pq, having no effect on the color of DCIP and thus remaining blue (oxidized form). The absorbance values indicate there was no significance in the results since there were no significance decreases in absorbance. The values were relative to one another; the values in between rounds fluctuated only about a range of 0.001 - 0.2. Since the absorbance values did not significantly decrease (meaning, a change in color of DCPIP from blue to clear), it can be hypothesized that herbicide stopped photosynthesis (specifically, affected the electron transport chain) before Pq. Pq plugged the electron transport chain by preventing DCIPIP from obtaining electrons. The support of Hypothesis 1 inherently refutes Hypothesis 2, meaning that herbicide stopped photosynthesis after Pq which resulted in DCIPIP changing color from blue to clear since it was able to steal electrons.
--------------------
I have identified 4 nominalizations.
My character(s) include my hypothesis being supported/refuted through experimental data.
The purpose of this paragraph is to support my hypothesis of the effect of herbicide on the process of photosynthesis.
Topic Position Stress Position (indicated by /)
1. The absorbance values indicate there was no significance in the results / since there were no significance decreases in absorbance.
2. The values were relative to one another; / the values in between rounds fluctuated only about a range of 0.001 - 0.2.
3. Pq plugged the electron transport chain / by preventing DCIPIP from obtaining electrons.
4. The support of Hypothesis 1 inherently refutes Hypothesis 2 - / meaning that herbicide stopped photosynthesis after Pq which resulted in DCIPIP changing color from blue to clear since it was able to steal electrons.
New paragraph: In this experiment, the effect of herbicide was tested on spinach leaf chloroplasts by measuring absorbance values during the process of photosynthesis. Two hypotheses were proposed: herbicide will affect the electron transport chain either before or after Pq. In order to test the hypothesis, 1 experiment cuvette and 4 controls – each containing different ratios of chloroplast solution, Na3PO4 (sodium phosphate buffer), herbicide, DCPIP (and reduced DCPIP), and water -- were tested. By examining the recorded absorbance data (Table 1), Hypothesis 1 was supported: herbicide stopped photosynthesis before Pq, having no effect on the color of DCIP and thus remaining blue (oxidized form). The absorbance values indicate there was no significance in the results since there were no significance decreases in absorbance. The values were relative to one another; the values in between rounds fluctuated only about a range of 0.001 - 0.2. Since the absorbance values did not significantly decrease (meaning, a change in color of DCPIP from blue to clear), it can be hypothesized that herbicide stopped photosynthesis (specifically, affected the electron transport chain) before Pq. Pq plugged the electron transport chain by preventing DCIPIP from obtaining electrons. The support of Hypothesis 1 inherently refutes Hypothesis 2, meaning that herbicide stopped photosynthesis after Pq which resulted in DCIPIP changing color from blue to clear since it was able to steal electrons.
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